Figure 2.
Effect of chebulagic acid on enterovirus 71 (EV71) replication in rhabdomyosarcoma (RD) cells. (A) The infected-RD cells were treated with chebulagic acid or saline at 2 h post EV71 infection, and then the cytopathic effect (CPE) of the RD cells were observed after AO/EB double staining under a light microscope (100×) at 0 h, 48 h and 72 h post infection, respectively; (B) The viral RNA copies in the culture supernatant of the RD cells were detected by quantitative RT-PCR (qRT-PCR). The data are expressed as the mean values of three independent experiments.