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Oligonucleotide primers employed to amplify SmugalU gene and to construct the gene coding for the SmuGalU-Δ294EcoGlgC chimeric protein. Restriction sites are underlined.
| Primer | Sequence | Restriction site |
|---|---|---|
| Smu1 | 5′-GGATCCCATGCCAAGTAAAAAAGTCAG-3′ | BamHI |
| Smu2 | 5′-GAATTCCTTAATCCGAGTTCTTTTGAG-3′ | EcoRI |
| Qmr1 | 5′-CTCGGACCCGGAACTGGATATGTACGATC-3′ | -- |
| Qmr2 | 5′-GTTCCGGGTCCGAGTTCTTTTGAGTCG-3′ | -- |
| Qmr3 | 5′-CTCGAGTTATCGCTCCTGTTTATGCCC-3′ | XhoI |
