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. 2013 May 7;14(5):9751–9766. doi: 10.3390/ijms14059751

Figure 1.

Figure 1

Figure 1

VEGF expression and production in ovarian epithelial carcinoma cells was induced by resistin. (A) Time course of 100 ng/mL resistin treatment on VEGF mRNA levels in HO-8910 cells; (B) Dose-response effect of 24-h resistin treatment on VEGF mRNA levels in HO-8910 cells; (C) Attenuation of resistin-induced VEGF expression by anti-resistin neutralizing antibody in HO-8910 cells. Cells were pretreated with or without anti-resistin neutralizing antibody (1:1000 or 1:250) for 1 h, then incubated in the presence of resistin 100 ng/mL for 24 h. Relative mRNA levels were normalized to that of untreated cells; (D) Dose-response effect of 24-h resistin treatment on VEGF peptide levels in the medium of HO-8910 cells; (E) Effects of resistin on VEGF promoter (pLuc 1) activity in HO-8910 cells. Cells were transfected with pLuc 1 together with control plasmid with use of jetPEI reagent. After transfection for 24 h, cells were stimulated with 100 ng/mL resistin or PBS, then luciferase activity was measured. Relative luciferase activity was normalized with respect to the activity in untreated cells. The results are representative of four independent experiments performed in triplicate. Data are means ± SEM. *p < 0.05; **p < 0.01 vs. untreated cells; #p < 0.05, ##p < 0.01 vs. resistin treatment alone.