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. 2013 May 7;14(5):9751–9766. doi: 10.3390/ijms14059751

Figure 2.

Figure 2

Figure 2

PI3K/Akt pathway was activated upon resistin stimulation. (A) Effects of resistin on PI3K/Akt pathway activation in HO-8910 cells. Cells were incubated with 100 ng/mL resistin for 1 h and whole-cell lysates underwent Western blotting to detect the phosphorylation of p85 subunit of PI3K and Akt (Ser 473); (B–D) Attenuation of resistin-induced VEGF mRNA expression (B), peptide secretion (C) and promoter activity (D) by LY294002 and wortmannin in HO-8910 cells. Cells were pretreated with (+) or without (−) LY294002 (20 μM) and wortmannin (2 μM) for 1 h, then incubated in the presence (+) or in the absence (−) of resistin 100 ng/mL for 24 h. Relative VEGF mRNA levels were normalized with respect to the levels for untreated cells. The basal promoter activity of each test plasmid is indicated as luciferase activity normalized by each internal control activity (PRL-TK). Relative luciferase activity was normalized with respect to the activity in untreated cells. The results are representative of four independent experiments performed in triplicate. Data are means ± SEM. **p < 0.01 vs. untreated cells, ##p < 0.01 vs. resistin treatment alone.