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. 2013 May 7;14(5):9751–9766. doi: 10.3390/ijms14059751

Figure 5.

Figure 5

HO-8910 cells-derived VEGF mediated resistin-induced angiogenesis of EA.hy926 cells. (A) Capillary-like tube formation assay of EA.hy926 cells co-cultured with HO-8910 cells under resistin (100 ng/mL) stimulation for 18 h. The total length of capillary-like tubes was measured and normalized with resistin-untreated EA.hy926 cells. Data are mean ± SEM from three independent experiments. ** p < 0.01 vs. resistin-untreated EA.hy926 cells only, ##P < 0.01 vs. resistin-treated EA.hy926 cells; (B) Blockage of VEGF by neutralizing antibody retarded capillary-like tube formation ability of EA.hy926 cells co-cultured with resistin-stimulated HO-8910 cells. The total length of capillary-like tubes was measured and normalized with controls. Data are mean ± SEM from three independent experiments. **p < 0.01 vs. untreated cells, ##p < 0.01 vs. resistin treatment alone; (C) Representative microscopic view of capillary-like tube formation in EA.hy926 cells co-cultured with HO-8910 cells under resistin (100 ng/mL) stimulation without or with VEGF neutralizing antibody treatment.