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Effect of Rg5 on DNA binding activities of NF-κB (A) and AP-1 (B) in LPS-stimulated BV2 cells. Nuclear extracts were prepared from BV2 cells after treatment with LPS (100 ng/mL) for 3 h in the absence or presence of Rg5, and then DNA binding activities were examined by electrophoretic mobility shift assay (EMSA). The data show that Rg5 significantly inhibited LPS-induced DNA binding activities of NF-κB and AP-1. The results are representative of three independent experiments.
