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. 2013 May 10;14(5):9999–10014. doi: 10.3390/ijms14059999

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(A) Optical microscope images after Prussian blue staining of SKBR-3 cells incubated with MNP-Abs; A-a, MCF-7 cells incubated with MNP-Abs; A-b, H520 cells incubated with MNP-Abs; A-c, and only SKBR-3 cells; A-d, (scale bar = 20 μm); (B) Fluorescent visualization by utilizing tyramide signal amplification generated by the intrinsic peroxidase activity of MNP-Abs applied to SKBR-3 cells; B-a, MCF-7 cells; B-b, and H520 cells; B-c, Upper and lower images are bright field optical images and cy5 fluorescence images, respectively (scale bar = 40 μm).

(A) Optical microscope images after Prussian blue staining of SKBR-3 cells incubated with MNP-Abs; A-a, MCF-7 cells incubated with MNP-Abs; A-b, H520 cells incubated with MNP-Abs; A-c, and only SKBR-3 cells; A-d, (scale bar = 20 μm); (B) Fluorescent visualization by utilizing tyramide signal amplification generated by the intrinsic peroxidase activity of MNP-Abs applied to SKBR-3 cells; B-a, MCF-7 cells; B-b, and H520 cells; B-c, Upper and lower images are bright field optical images and cy5 fluorescence images, respectively (scale bar = 40 μm).