Figure 1. Overview of GLT1a and GLT1b mRNA expression in brain and spinal cord.
GLT1 mRNA labeling in rat brain using probes covering the full length GLT1 coding sequence (GLT1pan, A, D, G, J), or specific stretches of the 3′-UTR sequence of GLT1a (B, E, H, K) and GLT1b (C, F, I, L). Regions shown are cerebral cortex and hippocampus (A–C), dorsal olfactory tubercle including the anterior olfactory nucleus (D–F), cerebellum (G–I), and spinal cord (J–L). GLT1a and GLT1b labeling patterns are similar and the combination of them equals the labeling seen with the GLT1pan probe. GLT1a is the predominant GLT1 mRNA expressed in the CA3 pyramidal cells in hippocampus (B, arrowhead) or the neurons of the dorsal olfactory nucleus (E, arrowhead). Generally GLT1a and GLT1b labeling in astrocytes appear similar in intensity, though Bergmann glia in cerebellum are more intensly labeled for GLT1a than for GLT1b. Inserts in G–I and J–L show magnifications of Bergmann glia (arrows in H, I) in cerebellum and astrocytes in the ventral horn, respectively. Bars: C, L 200 μm; F, I 100 μm.