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. Author manuscript; available in PMC: 2013 Jun 9.
Published in final edited form as: J Comp Neurol. 2005 Nov 7;492(1):78–89. doi: 10.1002/cne.20737

Figure 7. The GLT1b probe causes non-specific labeling at high concentration.

Figure 7

In situ labeling in hippocampus (A–F) and choroid plexus (G–J) produced by elevated (240 ng/ml) concentrations of probes against GLT1a (A, C, H), GLT1b (B, D, I), GLT1pan (G), and GLT1b sense (J), and by a low concentration (20 ng/ml) of GLT1a (E) and GLT1b (F). The high GLT1b probe concentration causes non-specific labeling in CA1 neurons (B, arrow) as well as other neurons, whereas the same concentration for GLT1a (or GLT1pan, not shown) does not label the CA1 neurons (A, arrow). Arrows in C–F point to the edge of the CA1 area at higher magnification. The high GLT1b probe labels the CA1 neurons non-specifically (D), which is not seen at the lower probe concentration (F). The GLT1a probe does not label the CA1 neurons at the high (C) or the low (E) probe concentration. The specific labeling of CA3 neurons by low GLT1b probe in F, which is not clearly seen at this magnification, is highlighted with an arrowhead in the insert. The high GLT1b probe also labels choroid plexus non-specifically (I, arrow), which is again not observed with GLT1pan (G), GLT1a (H), or GLT1b sense (J) probes. For this experiment, probes were purified over RNA columns and the concentrations were precisely measured with a Nanodrop spectrophotometer. Bars: B 250 μm, F 25 μm for inserts, J 50 μm (for C–J).