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. 2013 May 23;2013:413906. doi: 10.1155/2013/413906

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Copyright © 2013 Dong Hoon Kwak et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

HRT inhibits the Raf/MEK/ERK1/2 phosphorylation in early stage of 3T3-L1 adipocytes differentiation. Preadipocytes were induced to differentiate with HRT (200 μg/mL) and harvest at 30 min, 1 h, and 2 h and during the early stage of differentiation. Panels are indicating (a) the expression of ERK1/2, (b) the expression of c-Raf, and (c) the expression of Ras/MEK1. Panel is indicating (d) treatment of Erk phosphorylation inhibitor (PD98059, 50 μM) as a positive control for HRT treatment. The proteins were analyzed by western blot. Values are mean ± S.D. of data from three separate experiments; each experiment was performed in triplicate. **P < 0.01 versus control, and ***P < 0.001 versus control.