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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mucosal Immunol. 2012 Aug 8;6(2):309–323. doi: 10.1038/mi.2012.73

Figure 5.

Figure 5

FVB.mdr1a−/− CD4+Foxp3+ cells do not have higher co-expression of IFN-γ or IL-17 in the intestines. (a) Representative FACS plots of Foxp3 vs IFN-γ or Foxp3 vs IL-17A in spleen, MLN, PP, small intestinal LP, and colonic LP. Cells were isolated from tissues and rested overnight prior to stimulation with PMA and ionomycin in the presence of monensin. Cells were then stained for CD4, Foxp3, IFN-γ and IL-17. Lymphocytes were gated on based on forward scatter/side scatter and subsequently gated on CD4+ cells. (b) Dot plots representing the individual mice and the mean from one representative experiment. Data was reproduced in 3 separate experiments with 3 mice male mice (6–8 weeks of age) per group. Mean + standard deviation shown.