Figure 4.

Immunocytochemical analysis of costimulatory marker expressions on the cell surface of DCs. Co-stimulatory marker expression on the surface of DCs was measured by immuno-cytochemistry. Results were analyzed by ANOVA with Dunnett's multiple comparisons test, comparing all treatment categories to the infected-untreated control. Infected-untreated controls were compared to the untreated-uninfected control using the Student's t-test, *P ≤ 0.05, ***P ≤ 0.001. (a) Quantification of CD80 expression on DCs. (b) Quantification of CD86 expression on DCs. (c) Quantification of HLA-DR expression on DCs. (d)–(i) Fluorescent microscopy images of DCs from control and NAC-treated categories (20x magnification). Antibodies against HLA-DR and CD86 are labeled with FITC 488 (green) and PE 546 (red), respectively. (d) Bright field view of control DCs overlaid with fluorescent microscopy images using DAPI. (e) Control category DCs labeled with FITC conjugated anti- HLA-DR antibodies. (f) Control category DCs labeled with PE conjugated anti-CD-86 antibodies. (g) Bright field view of NAC treated DCs overlaid with fluorescent microscopy images using DAPI. (h) NAC treated DCs labeled with FITC conjugated anti- HLA-DR antibodies. (i) NAC treated DCs labeled with PE conjugated anti-CD-86 antibodies.