Table 4.
Determination of antimutagenic activity of Conyza extracts in Salmonella typhimurium TA1535 against sodium azide (NaN3; 2 μg/plate).
Treatment |
NaN3 mutagenicity at Conyza extract concentrations (revertant colonies/plate; mean ± SEM (% reduction in NaN3 mutagenicity)) |
|||
---|---|---|---|---|
Preexposure* | Coexposure* | |||
1 mg/mL | 5 mg/mL | 1 mg/mL | 5 mg/mL | |
NaN3 (2 μg/plate) | 290.3 ± 17.1 (0) | |||
I | 179.0 ± 10.5 (41)a | 31.3 ± 5.2 (95)a,b | 286.0 ± 18.0 (4) | 215.0 ± 18.1 (28)a,b |
II | 129.3 ± 4.2 (59)a | 11.3 ± 1.9 (102)a,b | 291.0 ± 21.5 (0) | 24.0 ± 4.7 (97)a,b |
III | 22.3 ± 2.9 (98)a | 13.3 ± 1.5 (101)a | 14.3 ± 2.7 (101)a | 10.0 ± 1.7 (103)a |
IV | 64.0 ± 5.7 (83)a | 18.7 ± 3.7 (99)a,b | 74.0 ± 4.1 (79)a | 20.7 ± 1.2 (99)a,b |
V | 10.7 ± 1.2 (102)a | 13.7 ± 1.5 (101)a | 58.7 ± 8.0 (85)a | 31.3 ± 6.1 (95)a |
*Plant extracts were incubated with bacteria either 30 minutes before NaN3 (Preexposure) or incubated with the bacteria and NaN3 (coexposure). The spontaneous revertant colonies were 17.0 ± 2.1. aSignificant (P < 0.05) reduction (% of inhibition of mutagenicity indicated in parentheses) from revertant colonies seen with NaN3. bSignificant difference (P < 0.05) between plant extract concentrations.