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. Author manuscript; available in PMC: 2013 Jun 9.
Published in final edited form as: ChemMedChem. 2012 Apr 23;7(6):1071–1083. doi: 10.1002/cmdc.201200102

Figure 7.

Figure 7

Lithocholic (●), isolithocholic (■) and cholanic acid (▲) dose-dependently inhibit Eph receptor phosphorylation. a) Inhibition of EphA2 phosphorylation. b) Inhibition of EphB4 phosphorylation. EphA2 phosphorylation was induced by 0.25 μg/ml ephrin-A1-Fc in PC3 cells, whereas EphB4 phosphorylation was induced with 3 μg/ml ephrin-B2-Fc, preclustered with 0.3 μg/ml anti-Fc antibodies in T47D cells. Cells were pretreated for 20 minutes with 1% DMSO, or the indicated concentrations (μM) of compounds and then stimulated for 20 minutes with ephrin-A1/-B2-Fc. Data are the means of at least three independent experiments ± standard error.