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Cholanic acid does not inhibit EphA2 kinase activity. The enzymatic activity of the recombinant human EphA2 kinase domain was evaluated with the LANCE® method using ATP and Ulight-TK peptide as substrate. EphA2 was incubated for 30 minutes with the indicated compounds at concentrations of 100 μM, 1 μM staurosporine or 1% DMSO as a control. **, p<0.01 for the comparison to control by one-way ANOVA followed by Tukey's multiple comparison test.
