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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Exp Gerontol. 2013 Mar 6;48(6):579–586. doi: 10.1016/j.exger.2013.02.027

Fig. 2.

Fig. 2

Decreased ability of Tg CD8 T cells of aged P14 mice to proliferate when cultured with specific antigen in the presence of splenocytes of young mice as APCs. 1 × 105 CFSE-labeled purified P14 CD8 T cells (Thy1.2+) were cultured with or without GP33-41, and 5 × 105 splenocytes from Thy1.1+ young B6 mice. (A). A representative experiment of proliferation of the TCR-Tg CD8 T cells (gated on Thy1.2+CD8+) was examined 4h through 72h after stimulation based on the profile of CFSE intensity. The experiment was performed with splenocytes of five individual aged and young P14 mice with similar results. (B). After stimulation with GP33-41, the in vitro proliferation data pooled from 5 independent experiments.