Figure 1.
TRAP1 Knockdown Inhibits In Vitro and In Vivo Neoplastic Transformation
(A and B) Human osteosarcoma SAOS-2 cells, human colorectal carcinoma HCT116 cells, and human cervix carcinoma HeLa cells lose the capability to form foci (A) or colonies in soft agar (B) after knocking down TRAP1 expression. Cells stably transfected with a scrambled shRNA or with TRAP1 shRNAs are dubbed mock and shTRAP1, respectively. Data indicate the total focus or colony area at the 25th experimental day. Representative areas showing focus or colony growth are reported.
(C) Rate of growth of mock and shTRAP1 SAOS-2, HCT116, and HeLa cells.
(D) Kinetics of tumor growth in nude mice after injection of SAOS-2 cells without or with a Matrigel bolus (left and right, respectively); representative tumors grown with Matrigel are shown on the right. Data are reported as mean ± SD values (n ≥ 3).