TABLE 3.

Pathway enrichment analysis of predicted and validated miRNA targets and differentially regulated transcripts in DGF and acute rejection

DGF	Predicted targets	Experimentally validated targets	Cleveland data set	Cleveland data set (ATN)
Pathways	P	P	P	P
Angiogenesis	<0.001	<0.001	0.015	0.035
Apoptosis signaling pathway	<0.001	0.008	0.014	0.011
TGF-ß signaling pathway	<0.001	<0.001	0.001	0.001
Endothelin signaling pathway	0.001	0.038	0.002	0.002
VEGF signaling pathway	0.011	<0.001	0.028	0.016
PDGF signaling pathway	<0.001	0.002	0.025	0.037

Acute rejection	Predicted targets	Experimentally validated targets	Cleveland data set (acute rejection)	ARTS
Pathways	P	P	P	P
EGFR signaling pathway	<0.001	0.014	0.001	0.012
Inflammation mediated by chemokine and cytokine  signaling pathway	<0.001	<0.001	0.039	<0.001
T-cell activation	0.003	0.044	<0.001	<0.001
Cytoskeletal regulation by Rho GTPase	0.001	0.011	0.005	0.047
B-cell activation	0.013	0.020	0.031	0.034

ARTS, acute rejection transcript set; ATN, acute tubular necrosis; DGF, delayed graft function; EGFR, epidermal growth factor receptor; miRNA, microRNA; PDGF, platelet-derived growth factor; TGF, transforming growth factor; VEGF, vascular endothelial growth factor.