Table 2.
The VL, VH families, H-CDR3s and subclasses of the selected antibodies
| Library | Clone | VL | VH | H-CDR3 | Subclass |
|---|---|---|---|---|---|
| M325 | 011 | Vκ1 | VH2 | ARLAVDTVMVQGYFDL | IgG1 |
| 1-1 | Vλ6 | VH3A | VRQSLDNYAYHLDY | IgG4 | |
| 1-5 | Vκ1 | VH2 | ARLAVDTVMVQGYFDL | IgG1 | |
| 1-7 | Vλ6 | VH6 | ARDEVTGTGVLDY | IgG1 | |
| I3 | Vκ1 | VH1 | ARDHVDTPMGLDY | IgG1 | |
| I4 | Vκ1 | VH1 | ARDHVDTPMGLDY | IgG1 | |
| M5 | Vλ6 | VH6 | ARQGYTHRDVLTRQKFYFYYMDV | IgG4 | |
| K530 | 2-1 | Vλ6 | VH3A | VRQSLDNYAYHLDY | IgG2 |
| 2-2 | Vκ1 | VH2 | ARLAVDTVMVQGYFDL | IgG1 | |
| 2-4 | Vκ1 | VH2 | ARLAVDTVMVQGYFDL | IgG1 |
DNA sequencing of the selected clones from one round of ribosome display from M325 and K530 libraries.