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. 2013 Jun 10;4:120. doi: 10.3389/fphys.2013.00120

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Copyright © 2013 Fantini, Yahi and Garmy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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The effect of cholesterol is specific. Mixed GM1/SM (1:1, mol:mol), GM1/POPC (1:1, mol:mol), or GM1/POPC/cholesterol (2:1:1, mol:mol:mol) were prepared and probed with Aβ5-16 (10 μM) injected in the aqueous subphase. The data show the evolution of the surface pressure as a function of time following peptide injection underneath GM1/POPC (full squares), GM1/POPC/cholesterol (open squares), or GM1/SM (open triangles) monolayers. Each experiment was performed in triplicate and one representative curve is shown (S.D. <10%).