Figure 5.
The LMO1 +57 kb element functions as a transcriptional enhancer in blood cells. (a) F0 transgenic mouse analysis of the LMO1 +57 region. Shown is a representative wholemount E11.5 embryo stained with X-Gal to determine the β-galactosidase expression pattern driven by the putative +57 enhancer. Histological sections of the same embryo confirm LacZ staining in circulating blood cells within the heart as well as the foetal liver. Wholemount images were taken at 2 × and tissue section images at 40 × magnification. (b) The promoter and enhancer elements are active in T-ALL cell lines. Luciferase reporter constructs were generated for the P1 and P2 promoter regions and the +57 putative enhancer region (LMO1 P1/luc, P2/luc and SV/luc/+57). These were stably transfected into LMO1-expressing, non-LMO1-translocated Jurkat cells alongside empty vector counterparts. All constructs showed greater activity than the empty vector (pGL2basic). Data shown are from three independent experiments, each performed in triplicate.