Skip to main content
. 2013 Jun 10;3:146. doi: 10.3389/fonc.2013.00146

Figure 3.

Figure 3

Wnt signaling in telomerase knock-out mESC with short telomeres and β-catenin interactors. (A) Immunoblot and quantification of β-catenin detected in cytosolic cell extracts of mESC treated with LiCl (30 mM) for 3 h. The intensity of β-catenin was normalized to the intensity of β-tubulin. The histogram represents the average (±standard deviation) of three independent experiments. (B) qRT-PCR to measure the levels of Axin2 mRNA transcript. WT and KO cells were treated with Wnt3a (100 ng/mL) for 3 days. The histogram represents the average (±standard deviation) of three independent qRT-PCR experiments, each performed with three replicates. (C) Transcriptional output of β-catenin activity measured using a TCF-driven luciferase reporter system. The histogram represents the average (±standard deviation) of the ratio of Renilla normalized firefly luciferase activity in Top versus Fop plasmid transfected cells of two independent experiments. Using a student’s t-test, no difference was noted in the presence or absence of Wnt3a (P = 0.223).