Figure 4.

(A) qRT-PCR to quantify mTert transcript in WT, KO, and KO cells re-transfected with inducible mTert under a Doxycyclin inducible promoter (see text for details). The histogram represents the average (±standard deviation) of three qPCR replicates. (B) Q-FISH to evaluate telomere lengthening in KO cells re-transfected with mTERT under the induction of Doxycyclin for 70 days. (C) Rescue of the level of cytosolic β-catenin in KO cells stably re-transfected with mTert under the control of an inducible promoter. Doxycycline (Dox) was used to induce mTert transcription. The results were verified with two independent protein extractions.