Table 1.
Results of IP-MS of β-catenin in WT and mTert−/− mESC.
| Accession ID | Description | Total peptides | Peptides quantified | Average intensity WT a.u. | Average intensity KO a.u. | P value | Ratio KO/WT |
|---|---|---|---|---|---|---|---|
| Gi|86262157|ref|NP_808386.2| | Hypothetical protein LOC239796 | 4 | 3 | 0.00343 | 0.00155 | 0.00037 | 0.45091 |
| Gi|124486588|ref|NP_001074475.1| | Sickle tail protein isoform c | 28 | 28 | 0.05906 | 0.02017 | 0.00154 | 0.34160 |
| Gi|40254129|ref|NP_258435.2| | Armadillo repeat protein deleted in velo-cardio-facial syndrome homolog | 17 | 17 | 0.06524 | 0.05290 | 0.01504 | 0.81088 |
| Gi|6755368|ref|NP_035426.1| | 40S Ribosomal protein S18 | 5 | 5 | 0.01326 | 0.00710 | 0.01607 | 0.53595 |
| Gi|31982755|ref|NP_035831.2| | Vimentin | 11 | 8 | 0.00262 | 0.00749 | 0.01611 | 2.85760 |
| Gi|31542151|ref|NP_038827.2| | Arginyl-tRNA-protein transferase 1 isoform 1 | 8 | 8 | 0.03619 | 0.06278 | 0.02867 | 1.73447 |
| Gi|112807186|ref|NP_766307.2| | GCN1 general control of amino acid synthesis 1-like 1 | 2 | 2 | 0.00068 | 0.00027 | 0.02990 | 0.40295 |
| Gi|110225370|ref|NP_031488.2| | Adenomatosus polyposis coli protein (APC) | 35 | 35 | 0.05596 | 0.09804 | 0.03139 | 1.75192 |
| Gi|79750409|ref|NP_075025.2| | Hamartin | 7 | 7 | 0.00687 | 0.01120 | 0.03649 | 1.63012 |
Nine proteins were differently represented in β-catenin immunoprecipitations of WT and mTert−/− mESC. The stringency criteria used to determine hits are described in Section “Materials and Methods.” Note that β-catenin was immunoprecipitated in WT and mTert−/− cells with a comparable efficiency [over 20 peptides in all IPs and peptide intensity ratio (KO/WT) of 0.97].