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. Author manuscript; available in PMC: 2013 Jun 10.
Published in final edited form as: J Immunol. 2009 Jul 27;183(4):2475–2483. doi: 10.4049/jimmunol.0900986

Figure 2. IDO activity is essential to activate Tregs after CpG treatment.

Figure 2

Mice were treated with 50μg CpGs (i/v, #1826) or control ODNs (#2138). After 24 hours, FACS-sorted splenic Tregs (5000/well) were added to readout assays containing 105 responder BM3 T cells and APCs from CBK mice. A. CBA or IDO1-KO mice were used as sources of Tregs. B. Tregs were obtained from mice pre-treated for 48 hours with pellets containing vehicle alone or pellets impregnated with IDO inhibitor (D-1MT) before CpG treatment. Thymidine incorporation was assessed after 72 hours. Control cultures containing no sorted Tregs yielded 46+/-1.3 (A) and 31+/-2.5 (b) ×103 CPM respectively. Dotted arrows highlight potent suppression mediated by Tregs from CpG-treated CBA IDO-sufficient mice. Data are the mean of triplicate cultures (+/- 1sd), and are representative of experiments performed on two or more occasions.