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. 2013 Jun 15;190(12):6303–6310. doi: 10.4049/jimmunol.1203030

FIGURE 2.

FIGURE 2.

Proteolytically cleaved HCII exerts antimicrobial effects. (A) Evaluation of antimicrobial activity of HLE, HCII, or HCIIa (HCII+HLE) against E. coli bacteria using a radial diffusion assay. Inhibition zones (as illustrated above graph) were measured and presented as means ± SD (n = 3). (B) Antimicrobial effect of HCII preparations against E. coli in viable count assays. Bacterial survival (%) is shown as mean values ± SEM (n = 3). (C) Analysis of E. coli treated with HCII or HLE-cleaved HCII in 10 mM Tris (pH 7.4) using electron microscopy. (D) Fluorescence microscopy analysis of E. coli bacteria subjected to HCII, HCII+HLE, and HLE in 10 mM Tris (pH 7.4). Permeabilization was assessed using the impermeant probe FITC. Top panel: Nomarski images; bottom panel: fluorescence microscopy images of the same view fields (original magnification ×100).