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. 2013 Jun 15;190(12):6303–6310. doi: 10.4049/jimmunol.1203030

FIGURE 5.

FIGURE 5.

HCII suppresses bacterial growth ex vivo and in vivo. (A) P. aeruginosa was grown in blood from HCII+/+or HCII−/− mice or from HCII−/− mice supplemented with 100 μg/ml HCII [HCII−/−(+)]. The number of CFU at t = 0 was 1.7 × 106. After incubation for 6 h, the number of CFU was evaluated [HCII+/+, n = 19; HCII−/−, n = 18; HCII−/−(+), n = 13]. (B–E) HCII+/+ and HCII−/− mice were infected i.p. with P. aeruginosa, and analyses were performed after 12 h. Bacterial counts (HCII+/+, n = 17; HCII−/−, n = 21) (B), cytokines in plasma (HCII+/+, n = 8; HCII−/−, n = 8) (C), and platelet counts (HCII+/+, n = 17; HCII−/−, n = 18) (D) in comparison with noninfected mice (HCII+/+, n = 9; HCII−/−, n = 8). (E) Determination of aPTT and PT coagulation times (HCII+/+, n = 7; HCII−/−, n = 8). (F) Bacterial counts in organs of HCII+/+ and HCII−/− mice s.c. infected with P. aeruginosa (HCII+/+, n = 13; HCII−/−, n = 15). In bar diagrams, data are presented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. P. aer, P. aeruginosa.