Fig. 11.

Cytoprotective effects of CORM-A1 against BBB dysfunction caused by glutamate and iGluR agonists. Confluent quiescent CMVEC grown on semipermeable membranes in Costar Transwells were exposed to glutamate (2 mM), in the absence or presence of CORM-A1 (50 μM) for 5 h. A: TEER was detected using a Millicel electrical resistance system. B: paracellular permeability of endothelial monolayers to 3-kDa dextran-conjugated Alexa Fluor 488 was detected after 5 h incubation with glutamate and expressed as the ratio of abluminal to luminal fluorescence values. Data represent the average of 6 experiments. Values are means ± SE. *P < 0.05, compared with the baseline control values. †P < 0.05, compared with glutamate alone.