Fig. 5.

iGluR agonists increase ROS production by CMVEC. Confluent quiescent CMVEC were incubated with: glutamate, agonists of N-methyl-D-aspartate (NMDA) receptors (NMDAR) NMDA and cis-ACPD; or agonists of AMPA/kainate receptors (non-NMDAR), AMPA, and kainic acid, for 1 h at 37°C. A: dose-dependent effects of glutamate and iGluR agonists (0.1–1.0 mM) on ROS production. B: comparison of maximal ROS-increasing potencies of glutamate (1.0 mM) and iGluR agonists (0.5 mM). ROS production was detected by accumulation of Ox-DHE fluorescent products and expressed as %control baseline values. Data represent the average of 4 experiments. Values are means ± SE. *P < 0.05, compared with the basal value.