Fig. 6.

Effects of iGluR agonists on DNA fragmentation and loss of cell contacts in CMVEC. Confluent quiescent CMVEC were incubated with 2 mM glutamate or 0.1 mM of iGluR agonists (NMDA, cis-ACPD, AMPA, and kainic acid) for 3 h at 37°C. Apoptosis was detected by DNA fragmentation (A) and expressed as %baseline values. Loss of cell contacts was detected by the number of detached floating cells and expressed as %baseline values. Data represent the average of 3 experiments. Values are means ± SE. *P < 0.05, compared with the basal values.