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. 2013 Apr;19(4):517–526. doi: 10.1261/rna.035733.112

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FIGURE 4. — Examples of fluorescence time traces of three different single vRNA molecules with heat-annealed tRNAlys3. Fluorescence signals from the donor (green) and acceptor (red) fluorophores after donor excitation; fluorescence of the acceptor after acceptor excitation (brown). (A) Zero FRET: The two fluorophores are colocalized but are too far away to display a FRET effect. (B) Constant FRET: The molecule displays high FRET efficiency. (C) Dynamic FRET: Fluorescence time traces of a molecule showing dynamical changes in the FRET efficiency.