MiR-181b overexpression inhibited cell proliferation, migration, and invasion in vitro. (A) Overexpression of miR-181b decreased U87 and U251 cell growth. (*) Significant difference when compared with the miR-NC group (P < 0.05). (B) Wound-healing assay in U87 and U251 cells overexpressing miR-181b. The wound gaps were photographed and measured. (*) Significant difference when compared with the miR-NC group (P < 0.05). (C) Transwell invasion assay of U87 and U251 cells overexpressing miR-181b. Cells in the bottom of the invasion chamber were fixed, stained, and photographed, and the absorbance at OD570 was read. (*) Significant difference when compared with the miR-NC group (P < 0.05).