FIGURE 7.

IGF-1R overexpression rescued the miR-181b overexpression phenotype. (A) Overexpression of IGF-1R increased levels of p-AKT, p-ERK1/2, and HIF-1α protein. (B) Overexpression of IGF-1R rescued VEGF mRNA expression inhibited by miR-181b. The VEGF mRNA level was normalized to that of GAPDH. (C,E) pReceiver-Lv105-IGF1R or pReceiver-Lv105-Negative Control transduced U87 cells stably expressing miR-NC or miR-181b. Forty-eight hours after transduction, cells were trypsinzed and seeded into 96-well plates or into the upper well of the invasion chamber. Cell proliferation and invasion assays were performed as described in Materials and Methods. (D) U87 cells stably expressing miR-NC or miR-181b were treated as above, and wounds were made. (*) Significant differences in VEGF levels between miR-NC and miR-181b treatment (P < 0.05). Hashes indicate significant difference in VEGF levels in U87/miR-181b cells with or without IGF-1R overexpression (P < 0.05).