FIGURE 1.

Capzb exon 9 is activated during myogenesis and has intronic ACUAA elements. (A) Phase contrast photographs of proliferating C2C12 or cells treated with low serum for 72 h to induce myogenesis. Multinucleate myotubes are indicated by arrowheads. (B) RT-PCR measurement of changes in Capzb exon 9 inclusion during differentiation using primers in exons 8 and 10 of the endogenous Capzb gene. Exon 9 inclusion increases during differentiation. (C) Alignment of intronic sequences downstream from the 5′ splice site bordering Capzb exon 9 from several vertebrates. The ACUAA elements are shaded and marked with asterisks.