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. 2013 Apr 2;21(6):1151–1159. doi: 10.1038/mt.2013.56

Figure 1.

Figure 1

TALEN targeting, nuclease architecture, and modification of COL7A1 gene. (a) COL7A1 TALEN target site in exon 14 of the COL7A1 gene on chromosome 3. The mutation at 1837 (CGA>TGA) is shown. (b) Core constituents of the nuclease complex: an N-terminal deletion of 152 residues of Xanthomonas TALEs, followed by the repeat domain, and a +63 C-terminal subregion fused to the catalytic domain of the FokI nuclease. (c) RVD base recognition. The RVDs NN, NI, HD, and NG bind guanine, adenine, cytosine, and thymine, respectively, and are color coded. (d) TALEN array binding to the COL7A1 gene target sequence. Colored bars represent the target-specific RVDs and correspond to the RVD:DNA code in c. TALEN expression platforms. (e) DNA plasmids with TALEN arrays in trans with gene expression controlled by the mini-CAGGs promoter and the bovine growth hormone polyadenylation signal. (f) mRNA species shown with TALEN architecture, 5′-7-methylguanosine cap (7 mG) and polyadenylation signal. RVD, repeat variable diresidue; TALEN, transcription activator-like effector nucleases.