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. 2013 Apr 2;21(6):1151–1159. doi: 10.1038/mt.2013.56

Figure 5.

Figure 5

TALEN-mediated gene editing resulting in normalized transcripts in primary fibroblasts. Uncorrected RDEB cell analysis showing the mutated base at (a) the 1837 locus and (b) the ApaI sensitive base. Non-targeted allele in TALEN-treated cells showing (c) presence of the mutation and (d) the absence of the ApaI-resistant base. Gene-edited allele in TALEN-treated cells showing (e) correction of the 1837 mutation and (f) the presence of the ApaI sensitive base. Immunofluorescence staining for type VII collagen protein. (g) Untreated RDEB fibroblasts showed a complete absence of detectable type VII collagen protein and (h) TALEN-corrected cells showed expression of type VII collagen. Antibody staining for each cell type was performed at the same time, and representative images were generated with identical microscopy settings. Blue, DAPI nuclear stain; red, type VII collagen. RDEB, recessive dystrophic epidermolysis bullosa; TALEN, transcription activator-like effector nucleases.