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. 2013 Jun 9;4:2041731413492741. doi: 10.1177/2041731413492741

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© The Author(s) 2013

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — mRNA expression of (a) Vcan, (b) Dcn, (c) Bgn, (d) Postn, (e) Tnc, (f) Thbs2 or Tsp2, (g) Adamts1, (h) Mmp8, (i) Mmp13, (j) Mmp2, (k) Mmp9, (l) Mmp14 or MT1-MMP, (m) Smad9 or Smad8, and (n) Scx genes at different days of FDL tendon graft healing at days 3, 7, 14, 21, 28, and 35. mRNA expression of (o) Mkx and (p) Tppp3 was also assessed at days 3, 21, and 35.

FDL: flexor digitorum longus.

Total RNA was extracted from tendon and surrounding tissue from six mice and processed for real-time RT-PCR. Gene expression was standardized with the internal β-actin control and then normalized by the level of expression at day 3 post surgery. Data are presented as the mean fold induction (over day 3 surgery) and ±SD; *p < 0.05 or less considered significantly different from day 3.