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. 2013 May 20;110(23):9493–9498. doi: 10.1073/pnas.1300578110

Fig. 4.

Fig. 4.

hypTM123,M206,M230Q is constitutively active. (A) Analysis of MetN, CysH, MetB, and FecD RNA levels in C600 hypT− cells carrying an empty pBAD22 (control) or expressing hypT, hypTM123,206,230Q, and hypTM123,206,230I, respectively, by qRT-PCR. Expression was induced by adding arabinose at time point zero. No HOCl was added. Shown are the results of one representative experiment. (B) Intracellular free-iron levels of C600 hypT− cells carrying an empty pBAD22 (control) or expressing hypT and hypTM123,M206, M230Q, as determined by EPR. Results are expressed as mean ± SD (n ≥ 3). (C) Viability assay of C600 recA− hypT− cells carrying an empty pBAD22 (control) or expressing hypT and hypTM123,206,230Q after treatment with 2 mM H2O2. Results are expressed as mean ± SD (n ≥ 11). (D) DNA binding of HypT, HypT-HOCl2.75 mM, M123,206,230Q, M123,206,230,284Q, M123,206,230I, and M123,206,230I-HOCl2.75mM purified from BL21(DE3) cells as determined by FA. Results are expressed as mean ± SD (n ≥ 3).