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Iron regulates expression of the SPI-2 ssaG gene in a PmrA-dependent manner. GFP activity from the ssaG-gfp transcriptional fusion was determined in wild-type Salmonella grown in N-minimal medium at pH 4.6 with 1 mM Mg2+ for 8 h with or without 100 μM Fe3+ (A); or in wild-type and pmrA Salmonella grown in N-minimal medium at pH 4.6 with 1 mM Mg2+ with various concentrations of iron (0.008, 0.04, 0.2, and 4 μM Fe3+) or without iron (B).
