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. 2013 May 20;110(23):9511–9516. doi: 10.1073/pnas.1307116110

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Fig. 3. — Immunolocalization of histone PTMs in the nuclei of MSNs. Immunolocalization of histone acetylation (Left) including H3 acetyl-lysine14 (acK₁₄) and acetyl-lysine18 (acK₁₈), H4 acetyl-lysine5 (acK₅), acetyl-lysine8 (acK₈), and acetyl-lysine12 (acK₁₂) and histone H3 methylation (Right) including lysine4 (H3K4) methylation (me_n) and lysine9 (H3K9) monomethylation (me), dimethylation (me₂), and trimethylation (me₃). DNA stained with DAPI (Center) and merged images (Right) are shown. Intense DAPI staining in large puncta corresponds to heterochromatin (usually less actively transcribed). (Scale bar, 5 µm.)