Fig. 5.
Larval behavior in the two-choice small-format paradigm. (A) Cognate odorants elicit a range of behavioral responses. Odorants were tested at a 10−2 dilution. Each bar represents RI ±SEM (n = 8). Responses differ; for example, the response to 2,5-dimethylpyrazine differs from the responses to the eight odorants with the greatest RIs (ANOVA followed by Bonferroni post hoc test; P < 0.05). (B) Extraction of odorants for assessment of gradients. The SPME fiber was inserted at the indicated positions and odorants were extracted with SPME. The disk contains odor at a dilution of 10−2. (C and D) Assessment of gradients with GC-MS at 2 min and 5 min for acetal (C) and 2,5-dimethylpyrazine (D). Areas under GC-MS peaks are indicated to allow comparisons of levels of a particular odorant at different positions in the gradient (n = 3).