CYP20-3 functions in the OPDA signaling pathway. (A) P. syringae (Pst DC3000) growth (shown as cfu/cm2 leaf area) in WT (Col-0), cyp20-3, and complementation (cyp20-3/CYP20-3) Arabidopsis plants at 4 d postinoculation (mean ± SD; n = 3). (B) Root length of WT (Col-0) and mutant [cyp20-3, cyp20-3/CYP20-3, coi1-1, and COI1 (COI1/coi1)] plants in the absence and presence of 15 µM OPDA at 5 d after germination (mean ± SD for n shown). (A and B) Different letters indicate statistically significant differences between genotypes (Tukey–Kramer honestly significant difference test on all pairs; α = 0.05). (C) Transcript quantification by quantitative RT-PCR of ORGs in OPDA-treated (black bars) and untreated (white bars) WT (Col-0) and mutant (cyp20-3, cyp20-3/CYP20-3, coi1-1, and opr3) plants. Values were normalized to the expression of POLYUBIQUITIN (means ± SD; n = 3).