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. Author manuscript; available in PMC: 2013 Jun 10.
Published in final edited form as: J Proteome Res. 2009 Feb;8(2):808–817. doi: 10.1021/pr7007913

Figure 4.

Figure 4

S490D mutation attenuates the occludin/ZO-1 interaction. (a) BREC lysates (1 mg) were incubated with his-tagged wt-hOcc413–522 or S490D-hOcc413–522. The occludin fragment was captured with IDA-Ni2+ ProBond Resin and complexes were washed followed by elution with imidazole. Protein was resolved by SDS-PAGE, and subjected to immunoblot analysis for the presence of ZO-1 using anti-ZO-1 rabbit polyclonal (α-ZO-1-rab) antibody. (b) As in (a) but the blots were analyzed with rat α-ZO-1. Shown are representative blots of 4 separate experiments.