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. Author manuscript; available in PMC: 2013 Jun 10.
Published in final edited form as: Cancer Discov. 2012 Jul 19;2(8):722–735. doi: 10.1158/2159-8290.CD-12-0014

Figure 5.

Figure 5

IDO-deficiency is associated with attenuated induction of IL6 during 4T1 tumor metastasis. (A) Evaluation of IDO1 protein levels by IP-Western blot analysis of lung tissue lysates from WT and Ido1−/− mice following orthotopic engraftment of 4T1 tumor cells at the time points in weeks indicated above each lane, [C; epididymis lysate positive control lane, M; molecular weight marker lane]. (B) Evaluation of kynurenine levels by liquid chromatography-tandem mass spectroscopy-based analysis of homogenized lung samples from WT and Ido1−/− mice following orthotopic engraftment of 4T1 tumor cells at the time points in weeks indicted for each lane. Means ± SEM (N ≥ 6) are graphed with significance relative to baseline determined by 1-way ANOVA with Dunn’s test (*; P < 0.05, **; P < 0.01). (C) IL6 level determinations from cytokine bead array immunoassay-based analysis of homogenized lung samples from WT and Ido1−/− mice following orthotopic engraftment of 4T1 tumor cells at the time points in weeks indicted for each lane. Means ± SEM (N ≥ 3) are graphed with significance relative to baseline determined by 1-way ANOVA with Dunn’s test (*; P < 0.05, **; P < 0.01).