Figure 7.

Single-wavelength stopped-flow measurements of the reaction of hDDC with L-Dopa at pH 6.0 and 15°C. Reaction of hDDC (20 μM) with L-Dopa (1 mM) was carried out at 15°C in 50 mM Bis-Tris-propane (pH 6.0). Time courses at 420 and 335 nm are reported. The solid lines are from fits to (7). The inset shows the dependence of k _obs for the increase in the intensity of the 420 nm band as a function of the final concentrations of L-Dopa after mixing. The points shown are the experimental values, while the curve is from fit to the data using (8).