Table 1. Yeast strains used in this study.
Strain name | Strain genotype | Origin |
W303-1a | MATa leu23/112 ura31 trp11 his311/15 ade21 can1100 GAL SUC2 | Thomas and Rothstein (1989) [45] |
YMR84 | W303-1A with gpd1Δ::URA3 | Martijn Rep (Amsterdam)* |
YSH1583 | W303-1A with pfk26Δ::KanMX | This study |
YSH1585 | W303-1A with pfk27Δ::KanMX | This study |
YSH1586 | W303-1A with pfk26Δ::KanMX pfk27Δ::KanMX | This study |
YMT101 | W303-1A with fsp1Δ::LEU2 Ylp-URA3-fps1-Δ1 | Tamas et al., (1999) [8] |
YSH2293 | W303-1A with stl1Δ::KanMX | This study |
YSH444 | W303-1A with hog1Δ::TRP1 | Albertyn et al,. (1994) [12] |
W303-1A with hog1-att | This study |
*Strain YMR84 was kindly provided by Martijn Rep (Amsterdam) and contains a replacement of the GPD1 upstream region (−883 to +91) by the URA3 gene. The strains was generated using a PCR approach and does not express the GPD1 gene product.