Table 2.
Primers used for real-time RT-PCR.
| Target gene | Sequence | Length (bp) | |
|---|---|---|---|
| MAFbx/atrogin-1 | S AS |
5′-GGCGGACGGCTGGAA-3′ 5′-CAGATTCTCCTTACTGTATACCTCCTTGT-3′ |
100 |
| MuRF-1 | S AS |
5′-ACGAGAAGAAGAGCGAGCTG-3′ 5′-CTTGGCACTTGAGAGAGGAAGG-3′ |
179 |
| GAPDH | S AS |
5′-CGTGTTCCTACCCCCAATGT-3′ 5′-ATGTCATCATACTTGGCAGGTTTCT-3′ |
74 |
AS: antisense primer; S: sense primer; MAFbx/atrogin-1: muscle atrophy F-box protein; MuRF-1: muscle-specific RING finger protein 1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.