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. 2013 May 25;9(5):521–530. doi: 10.7150/ijbs.4962

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Cell proliferation of NIH3T3 and chondrocytes in 2-D and 3-D culture conditions. HG is poly (hydroxyethyl methacrylate)-gelatin cryogel and AG is alginate-gelatin cryogel. (+ve) indicates experiment with α-KG and (-ve) indicates experiment without α-KG. (A) The cellular proliferation of NIH3T3 was measured by (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The NIH3T3 cells were seeded on two different cryogel scaffolds HG and AG in 3-D along with the 2-D culture. The experiment was divided in two sets; one containing α-KG and the other without α-KG which were analyzed in regular time intervals. The diagram indicates cell proliferation for the samples containing α-KG compared to samples without α-KG. (B) Similar experiment was performed for primary chondrocytes. The graph indicates proliferation of chondrocyte in 3-D and 2-D in presence of α-KG as compared to the cells cultured without α-KG. All the experiments were performed in triplicates.