Figure 6.

Glucose metabolism during chondrocyte culture in 2-D and 3-D culture conditions in the presence and absence of α-KG. HG is poly (hydroxyethyl methacrylate)-gelatin cryogel and AG is alginate-gelatin cryogel. (+ve) indicates experiment with α-KG and (-ve) indicates experiment without α-KG. Effect of α-KG on the chondrocyte was checked over a period of 3 weeks in two different cryogel scaffolds. Standard graph was obtained by plotting the known concentration of glucose against the absorbance which was further used to determine the glucose concentration in the test samples. All the experiments were performed in triplicates.