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. Author manuscript; available in PMC: 2014 Apr 17.
Published in final edited form as: Bioconjug Chem. 2013 Apr 2;24(4):658–668. doi: 10.1021/bc300632w

Figure 4.

Figure 4

Cellular uptake of Rhodamine-labeled HA and CHA nanogels. (A) Confocal microscopy of: (a) MDA-MB-231/F cells incubated with Rh-CHA for 2 h at 37°C. (b) MDA-MB-231/F cells incubated with Rh-CHA for 2 h at 4°C. (c) MDA-MB-231/F cells pretreated with free HA and then incubated with Rh-CHA for 2 h at 37°C. (d) MDA-MB-231/F cells incubated with Rh-HA for 2 h at 37°C. (e) MCF-7 cells incubated with Rh-CHA for 2 h at 37°C. (B) CD44 expression in MDA-MB-231/F, MCF-7 and MIA PaCa-2 cells determined by flow cytometry. MFI: mean fluorescence index. (C) Quantitative comparison of the cellular uptake of fluorescent Rh-CHA measured per 50,000 cells: (a) at 4°C vs. 37 °C, (b) with or without pretreatment by HA, (c) HA vs. CHA, and (d) MCF-7 vs. MDA-MB-231/F. Data are expressed as means ± SEM.